"Pulse points" are those points on the body where you feel your heart beat--wrists, throat, under the ears, on the inner elbows and behind the knees. The veins are closest to the surface of the skin at these points. When the heart beats, the pulse disturbs the surface of the skin.
In the same way, the pulse beating at these points will disturb the fragrance you have dabbed or sprayed on. As the fragrance is disturbed, it is continuously refreshed. The pulse points are also warmer than other areas of the body. A perfume reacts to warmth and the scent is stronger on the warmer areas of a body, so if you spray or dab perfume on these warmer pulse points, it will make a stronger presence.
Because perfume (not eau de toilette) is so expensive, you want to make it go as far as you can. You also don't want to overuse a scent; you will overpower people as you encounter them. Using pulse points helps you to use less scent, which will subtly announce your presence.
If you have dry skin, get into the habit of "layering" your scent. Use a skin moisturizer of the same scent (or unscented) which you plan to wear. The moisturizer will keep skin moist, which means the perfume will stick to skin and last longer throughout the day. Apply moisturizer to the pulse points you will be using before you spray or dab on perfume. If your skin is oily, fragrance will last longer on your skin and pulse points than it will on someone who has dry skin. The scent you use will also be more intense because your skin is oilier. You will not need to reapply your scent as often as a woman with drier skin.
Do not rub perfume into skin. Doing so will break down some of the molecular composition (or "bruise") the perfume. Also, vary the pulse points from time to time. If you used your throat, behind your ears and your wrists, next time, use the crooks of your elbows and the backs of your knees.