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Papain is an enzyme found in the liquid of unripe papayas. In alternative medicine, it has been used to reduce inflammation and pain, and to speed up recovery of certain injuries, especially uncomplicated athletic injuries. Papain must be extracted before its potency reaches levels high enough to help with these conditions.

Cut the papaya from top to bottom with the razor blade. Make sure the cut is no more than 2mm deep.

Hold the papaya over the collection dish and let the liquid drip out onto the dish. This should be done for a minimum of six minutes. Do not squeeze the papaya to get the liquid out.

Place the collection dish into the vacuum oven. Set the vacuum oven to 104 degrees Fahrenheit. Let the liquid papain dry for five hours. You will know it is ready when it takes on a crumbly appearance.

Transfer the dried papain into a box that has been lined with polyethylene. This can be done by gently scraping the dried papain from the collection dish with a stainless steel spoon.The box must have a lid, which should be as airtight as possible. The box should be stored in a cool, dry place.


Multiple extractions can be done on the same piece of fruit. You can repeat this process every five to eight days. More than one cut might be required in later attempts.

It is best to do the extraction process in the morning as the increased humidity improves flow.


Papain is closely related to latex so if you have a latex allergy, you might also have an allergic reaction to the papain.

Non-stainless steel metal will ruin the papain extraction process.

About the Author

Camira Bailey

Camira Bailey has been writing for various online publications since 2006, specializing in health and animal care. She holds a Bachelor of Science in biology from UCLA and is completing her master's degree in holistic health. Bailey is also an ACE-certified advanced health and fitness specialist.